FZD7 antibody (Cysteine-Rich Domain, Extracellular) (Biotin)

Catalog No. ABIN393087

This anti-FZD7 antibody is a Rat Monoclonal antibody detecting FZD7 in ELISA, IHC and FACS. Suitable for Human and Mouse.

Quick Overview for FZD7 antibody (Cysteine-Rich Domain, Extracellular) (Biotin) (ABIN393087)

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FZD7 antibody is conjugated to Biotin

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-FZD7 Antibody (Biotin)

Binding Specificity: Cysteine-Rich Domain, Extracellular

Specificity: Recognizes mouse Frizzled-7. Shows no cross-reactivity with rmFrizzled-1, -3, -4 or -8. Species cross-reactivity: human.

Purification: Protein G purified

Immunogen: NS0-derived rmFrizzled-7 extracellular CRD.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, Flo, IHC

Usage: Suitable for use in Flow Cytometry, Immunohistochemistry and Direct ELISA. Detects Frizzled-7 by Flow Cytometry and Immunohistochemistry on the differentiated embryoid bodies derived from mouse embryonic cell line D3. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. Binding may be visualized by adding streptavidin conjugated to a fluorochrome. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.2, BSA

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for FZD7

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Alternative Name: FZD7 / Frizzled 7

Background: Name/Gene ID: FZD7
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD7, Frizzled family receptor 7, Frizzled homolog 7, Frizzled 7, Frizzled-7, FzE3, Fz-7, HFz7

Gene ID: 8324

UniProt: O75084

Pathways: WNT Signaling, Stem Cell Maintenance