DDX3X antibody (N-Term)

Catalog No. ABIN396914

The Rabbit Polyclonal anti-DDX3X antibody has been validated for WB, ELISA, IHC and IHC (p). It is suitable to detect DDX3X in samples from Human, Mouse and Rat.

Quick Overview for DDX3X antibody (N-Term) (ABIN396914)

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX3X antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DDX3X Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Synthetic peptide corresponding to N-terminal residues of human DDX3X (DEAD-box protein 3, X- chromosomal)

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human DDX3X.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (20 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.01 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for DDX3X

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Alternative Name: DDX3 / DDX3X

Background: Name/Gene ID: DDX3X
Family: DEAD box protein

Synonyms: DDX3X, CAP-Rf, DDX14, DDX3, DEAD box, X isoform, DBX, Dead box protein 3, DEAD/H box-3, Helicase like protein 2, Helicase-like protein 2, HLP2

Gene ID: 1654

UniProt: O00571

Pathways: Ribonucleoprotein Complex Subunit Organization, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Ribosome Assembly