alpha 2 Macroglobulin antibody (C-Term)

Catalog No. ABIN396916

This Rabbit Polyclonal antibody specifically detects alpha 2 Macroglobulin in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human and Rat.

Quick Overview for alpha 2 Macroglobulin antibody (C-Term) (ABIN396916)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha 2 Macroglobulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-alpha 2 Macroglobulin Antibody

Binding Specificity: C-Term

Specificity: Synthetic peptide corresponding to C-terminal residues of human A2M (Alpha-2-macroglobulin precursor)

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human A2M / Alpha-2-Macroglobulin.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.01 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: Alpha-2-Macroglobulin / A2M

Background: Name/Gene ID: A2M

Synonyms: A2M, CPAMD5, A2MD, FWP007, Alpha-2-M, Alpha-2-macroglobulin, S863-7

Gene ID: 2

UniProt: P01023

Pathways: Lipid Metabolism