Ubiquilin 1 antibody (Internal Region)

Catalog No. ABIN396937

This Mouse Monoclonal antibody specifically detects Ubiquilin 1 in WB, ELISA, IHC, IHC (p) and IP. It exhibits reactivity toward Human.

Quick Overview for Ubiquilin 1 antibody (Internal Region) (ABIN396937)

Target: Ubiquilin 1 (UBQLN1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquilin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: 3D5E2

Product Details anti-Ubiquilin 1 Antibody

Binding Specificity: Internal Region

Brand: IHC-plus™

Specificity: Specific for the ubiquilin protein. On Western blots, it identifies a band at ~66kD. Reactivity has been confirmed with human HEK293 cell lysates.

Purification: Protein A purified

Immunogen: Recombinant protein derived from an internal region of human ubiquilin.

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (10 μg/mL), IP, WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Immunoprecipitation: 2 μg/reaction. Use native protein.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Ubiquilin 1

Target: Ubiquilin 1 (UBQLN1)

Alternative Name: UBQLN1 / Ubiquilin

Background: Name/Gene ID: UBQLN1

Synonyms: UBQLN1, DA41, HPLIC-1, PLIC-1, Ubiquilin, UBQN, XDRP1, DSK2, PLIC1, Ubiquilin 1, Ubiquilin-1

Gene ID: 29979

UniProt: Q9UMX0