The Mouse Monoclonal anti-SIV Nef antibody is suitable to detect SIV Nef in samples from Simian Immunodeficiency Virus (SIV). It has been validated for IF, WB and ELISA.
Immunofluorescence (IF), Western Blotting (WB), ELISA
Clone
17
Binding Specificity
AA 71-80
Sequence
PAAEREKLAY
Specificity
Reacts in ELISA, WB, RIP, IHC with Nef of SIVmac251strain. Reacts in IF with Nef of SIVmac239 strain and SIVmac1A11strain. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format. SIV-1
Purification
purified with Protein G affinity chromatorgaphy
Immunogen
Recombinant SIV-1 Mac251 Nef protein of (purified fromE. coli)