EGFR antibody (pTyr1197)

Catalog No. ABIN400879

This Mouse Monoclonal antibody specifically detects EGFR in WB, FACS and IP. It exhibits reactivity toward Human.

Quick Overview for EGFR antibody (pTyr1197) (ABIN400879)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: EM-13

Product Details anti-EGFR Antibody

Binding Specificity: pTyr1197

Specificity: The antibody EM-13 reacts with EGFR (ErbB1 / HER1) phosphorylated on tyrosine 1197.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

Purification: Protein-A affinity chromatography

Purity: > 95 % pure by SDS-PAGE

Immunogen: Synthetic phospho-peptide covering sequence around tyrosine 1197 of human EGFR

Isotype: IgG1

Application Details

Application Notes: Flow cytometry. Immunoprecipitation. Western blot: 1 μg/mL. Positive control: EGF-stimulated A431. Negative control: Non-stimulated A431.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C for up to one month or at -20 °C for longer. Avoid repeated freezing andthawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR / ERBB1

Background: The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins