Biotin antibody (FITC)

Catalog No. ABIN401080

This Rabbit Polyclonal antibody specifically detects Biotin in FACS, IF and IHC (fro). It exhibits reactivity toward .

Quick Overview for Biotin antibody (FITC) (ABIN401080)

Target: Biotin

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Biotin antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Biotin Antibody (FITC)

Characteristics: Absorption / Emission: 495 nm / 528 nm
Molar Ratio: 2.7 Moles Fluorescein (FITC) per mole of IgG

Immunogen: Biotin conjugated Keyhole Limpet Hemocyanin (KLH)

Isotype: IgG

Application Details

Application Notes: Immunofluorescence. Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 10.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.01 M Sodium Phosphate, 0.11 M Sodium Chloride, pH 7.4, 0.01 % (w/v) Thimerisol, 10 mg/mL BSA (IgG and Protease free)

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background: Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.