L1/ORF2 antibody

Catalog No. ABIN401095

The Chicken Polyclonal anti-L1/ORF2 antibody is suitable to detect L1/ORF2 in samples from Chimpanzee and Human. It has been validated for EIA and WB.

Quick Overview for L1/ORF2 antibody (ABIN401095)

Target: L1/ORF2

Reactivity: Chimpanzee, Human

Host: Chicken

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Product Details

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee.
Species reactivity (tested):Human.

Purification: IgY fraction antibody purified from monospecific Chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer.

Immunogen: Two Synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10000. Western Blot: 1/2000 - 1/10000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details

Target: L1/ORF2

Alternative Name: L1 / ORF2

Background: The L1 (LINE-1), or Long INterspersed Element, retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retrotransposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons.Synonyms: LINE-1