FITC antibody

Catalog No. ABIN401128

This Goat Polyclonal antibody specifically detects FITC in WB, IHC (fro) and EIA.

Quick Overview for FITC antibody (ABIN401128)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Host: Goat

Clonality: Polyclonal

Conjugate: This FITC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-FITC Antibody

Purification: Immunoaffinity Chromatography.

Immunogen: Fluorescein conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/5,000-1/30,000. Western Blot: 1/500-1/3,000. Immunohistochemistry: 1/250-1/1,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Alternative Name: FITC

Target Type: Chemical

Background: Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is? pH sensitive and is significantly reduced below? pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.Synonyms: Fluorescein Isothiocyanate