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R-Phycoerythrin antibody (TRITC)

The Goat Polyclonal anti-R-Phycoerythrin antibody has been validated for FACS and IF. It is suitable to detect R-Phycoerythrin in samples from .
Catalog No. ABIN401162

Quick Overview for R-Phycoerythrin antibody (TRITC) (ABIN401162)

Target

R-Phycoerythrin (Phycoerythrin, R-)

Host

  • 11
  • 3
Goat

Clonality

  • 14
Polyclonal

Conjugate

  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
This R-Phycoerythrin antibody is conjugated to TRITC

Application

  • 8
  • 7
  • 6
  • 5
  • 3
  • 3
  • 3
  • 2
  • 2
Flow Cytometry (FACS), Immunofluorescence (IF)
  • Characteristics

    Absorption / Emission: 550 nm / 570 nm
    Molar Ratio: 20 Moles TRITC per mole of Goat IgG

    Purification

    Immunoaffinity Chromatography.

    Immunogen

    Highly purified R-Phycoerythrin from the seaweed gracila

    Isotype

    IgG
  • Application Notes

    Immunofluorescence. Flow Cytometry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore with 1.0 mL of deionized water (or equivalent).

    Concentration

    1.0 mg/mL (by UV absorbance at 280 nm)

    Buffer

    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Thimerosal as preservative.

    Preservative

    Thimerosal (Merthiolate)

    Precaution of Use

    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target

    R-Phycoerythrin (Phycoerythrin, R-)

    Alternative Name

    R-Phycoerythrin

    Target Type

    Chemical

    Background

    Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.Synonyms: rpeB

    Gene ID

    2775

    UniProt

    Q7SIF9
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