ATAD5 antibody (AA 63-76)

Catalog No. ABIN401276

The Rabbit Polyclonal anti-ATAD5 antibody has been validated for WB and EIA. It is suitable to detect ATAD5 in samples from Human.

Quick Overview for ATAD5 antibody (AA 63-76) (ABIN401276)

Target: ATAD5 (ATPase Family, AAA Domain Containing 5 (ATAD5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATAD5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ATAD5 Antibody

Binding Specificity: AA 63-76

Specificity: This antibody is directed against Elg1 protein.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 63-76 of Human Elg 1.

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 3,000 - 1: 15,000. Western blot: 1: 500 - 1: 2,000 (Expect a band approximately 120 kDa in size correspondingto Elg1 in the appropriate cell lysate or extract).

Restrictions: For Research Use only

Handling

Concentration: 0.50 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for ATAD5

Target: ATAD5 (ATPase Family, AAA Domain Containing 5 (ATAD5))

Alternative Name: ATAD5

Background: ELG1 (also known as ATP(GTP)-binding protein or Chromosome fragility associated gene 1) is involved in a novel RFC complex that is probably involved in DNA damage and repair by ensuring replication fidelity. This antibody detects a band at about 120 kDa in Hela, A431, Jurkat and HEK193 cells. This corresponds to the band size seen in Kanellis P et al. It remains unclear why the band size detected is much less than the 207 kDa predicted in the protein sequence corresponding to CACC44537.2 (Q96QE3), but as our results correspond to those seen in Kanellis P et al. it is likely that the 120 kDa target is ELG1.Synonyms: ATPase family AAA domain-containing protein 5, C17orf41, Chromosome fragility-associated gene 1 protein, FRAG1

Gene ID: 79915

NCBI Accession: NP_079133

UniProt: Q96QE3