DIAPH2 antibody (Isoform 2)

Catalog No. ABIN401289

This anti-DIAPH2 antibody is a Rabbit Polyclonal antibody detecting DIAPH2 in WB and EIA. Suitable for Human and Mouse.

Quick Overview for DIAPH2 antibody (Isoform 2) (ABIN401289)

Target: DIAPH2 (Diaphanous Homolog 2 (DIAPH2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIAPH2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-DIAPH2 Antibody

Binding Specificity: AA 1085-1101, Isoform 2

Specificity: This antibody is directed against DIA-2. This antibody will only react with DIA-156 (isoform 1 of DIA-2).

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding aa 1085-1101 of Human DIA-2

Isotype: IgG

Application Details

Application Notes: ELISA: 1/12,000 - 1/60,000. Western Blot: 1/1,000 - 1/6,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.4 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for DIAPH2

Target: DIAPH2 (Diaphanous Homolog 2 (DIAPH2))

Alternative Name: DIAPH2

Background: DIA-2 (also called DIA drome, Dia2, DIAPH2, Diaphanous 2, Diaphanous related formin 2, Diaphorase 2, DRF2, POF and POF2) may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3' UTR. Overall 4 splice variants have been described. DIA-2 is expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes and can be found from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles. Isoform 1 is referred to as DIA-156, whereas isoform 2 is called DIA-12C.Synonyms: DIA, DRF, Diaphanous-related formin-2, diaphanous homolog 2

Gene ID: 1730

NCBI Accession: NP_006720

UniProt: O60879