PDCD4 antibody (pSer457, Ser457)

Catalog No. ABIN401369

The Rabbit Polyclonal anti-PDCD4 antibody is suitable to detect PDCD4 in samples from Human, Mouse, Rat and Xenopus laevis. It has been validated for WB, IHC (p) and EIA.

Quick Overview for PDCD4 antibody (pSer457, Ser457) (ABIN401369)

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reactivity: Human, Mouse, Rat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDCD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PDCD4 Antibody

Binding Specificity: pSer457, Ser457

Specificity: This antibody is specific for Pdcd4 protein phosphorylated at Ser457.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Xenopus.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids surrounding Ser457 in the human Pdcd4 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/400,000. Western Blot: 1/500 - 1/2,000. Immunohistochemistry: 21.25 - 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.95 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Alternative Name: PDCD4

Background: Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4

Gene ID: 27250

NCBI Accession: NP_055271

UniProt: Q53EL6