POGZ antibody (N-Term)

Catalog No. ABIN401435

The Rabbit Polyclonal anti-POGZ antibody is suitable to detect POGZ in samples from Human, Mouse, Rat, Dog, Cow, Chicken, Macaque, Olive Baboon and Chimpanzee. It has been validated for WB and EIA.

Quick Overview for POGZ antibody (N-Term) (ABIN401435)

Target: POGZ (Pogo Transposable Element with ZNF Domain (POGZ))

Reactivity: Human, Mouse, Rat, Dog, Cow, Chicken, Macaque, Olive Baboon, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POGZ antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-POGZ Antibody

Binding Specificity: N-Term

Specificity: This antibody is known to react with Pogz protein.

Cross-Reactivity (Details): Species reactivity (expected):Human, Canine, Cattle, Rat, Chimpanzee, Macaque, Olive Baboon, Chicken, Short-tailed opossum.
Species reactivity (tested):Mouse.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to a region near the N-terminus of mouse Pogz protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/150,000. Western Blot: 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.564 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for POGZ

Target: POGZ (Pogo Transposable Element with ZNF Domain (POGZ))

Alternative Name: POGZ

Background: Pogz is a zinc-finger protein containing at least 8 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family. Members of this family of integrases share structural homologies and show similar catalytic activity to the RAG1 and RAG2 proteins that initiate V(D)J recombination in lymphoid progenitors. Mouse Pogz has a human homolog with a base pair similarity of 90 % and amino acid similarity of 93 % for the full length protein. In humans at least 3 variants have been predicted and they are all similar at the N-terminus. A number of expressed sequence tags (ESTAs) cloned from murine undifferentiated ES cells and Lin-/c-Kit+/Sca-1+ hematopoietic stem cell cDNA libraries correspond to the Pogz gene, further underlining an important function for this gene in stem cells. In mice, deletion of the gene in all tissues early in embryogenesis has been shown to be lethal.Synonyms: KIAA0461, Pogo transposable element with ZNF domain, SUHW5, Suppressor of hairy wing homolog 5, ZNF280E, ZNF635

Gene ID: 229584

NCBI Accession: NP_766271

UniProt: Q8BZH4