OCT4 antibody (Internal Region)

Catalog No. ABIN401447

The Rabbit Polyclonal anti-OCT4 antibody is suitable to detect OCT4 in samples from Human, Mouse, Horse, Chimpanzee and Macaque. It has been validated for WB and EIA.

Quick Overview for OCT4 antibody (Internal Region) (ABIN401447)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human, Mouse, Horse, Chimpanzee, Macaque

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-OCT4 Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts with Oct-4 protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Macaque, Horse, Mink, Canine, Bovine, Porcine.
Species reactivity (tested):Human, Mouse.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to an internal region of human Oct-4 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/350,000. Western blot: 1/1,000 - 1/1,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: OCT3/4

Background: Oct-4, also known as POU domain transcription factor, Octomer- binding transcription factor 3 and Oct-3, is expressed in human and mouse totipotent embryonic stem and germ cells. It may function in preventing these cells from differentiating. Each type of established mammalian pluripotent stem cell line (embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells) expresses Oct-4, which disappears rapidly when the cells differentiate. Most studies on Oct-4 have been performed on murine embryos. In human oocytes of various maturity and normally developing preimplantation/cleavage embryos, a variable Oct-4 expression pattern has been revealed, concomitant with a pure cytoplasmic localization of the protein. This variability in expression fades away during compaction, indicating embryonic Oct-4 expression, the protein appears in the nucleus, implying biological activity. Functional Oct-4 has been found in human amniotic fluid. As Oct-4 transcripts have also been found in various differentiated cells, the presence of Oct-4 transcripts or proteins may not be sufficient for identifying undifferentiated cell lines in humans. It may also be important to examine the localization of Oct-4 proteins within a cell. Oct-4 has been shown to interact with Nanog and with proteins from multiple repression complexes, including the NuRD, Sin3A and Pml complexes, possibly to control ES fate. Reactivation of Oct-4 expression is postulated to occur in differentiated cells that have undergone carcinogenesis, or tumor formation.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1

Gene ID: 5460

NCBI Accession: NP_002692

UniProt: Q01860

Pathways: Stem Cell Maintenance