Recombinant RFP antibody (Atto 488)

Catalog No. ABIN4368335

This anti-RFP antibody is a Alpaca Monoclonal antibody detecting RFP in IF, ICC and FACS. Suitable for Discosoma.

Quick Overview for Recombinant RFP antibody (Atto 488) (ABIN4368335)

Target: RFP (Red Fluorescent Protein (RFP))

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Discosoma

Host: Alpaca

Clonality: Monoclonal

Conjugate: This RFP antibody is conjugated to Atto 488

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 2B12-2A1

Product Details anti-RFP Antibody (Atto 488)

Expression System: E.coli

Purpose: Camelid sdAb anti-RFP conjugated with Atto488, Clones 2B12/2A1

Specificity: Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.

Cross-Reactivity (Details): Does not cross-react with GFP or mTagBFP derivatives.

Characteristics: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Purification: Produced in: E.coli

Immunogen: RFP

Labeling Ratio: A blend of 2 different sdAbs that bind at different epitopes of the same target protein, each sdAb bears 2 site-specifically coupled fluorophores.

Application Details

Application Notes: Recommended dilution 1:500

Comment:

A blend of 2 different sdAbs that bind at different epitopes of the same target protein, two fluorophore molecules are site-specifically coupled to each individual sdAb molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this product can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render this products excellent tools for all microscopy techniques.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Concentration: 5 μM

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Handling Advice: Protect from light!

Storage: -20 °C,-80 °C

Storage Comment: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Expiry Date: 12 months

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: RFP

Molecular Weight: 26 kDa