HBSAg antibody

Catalog No. ABIN452186

The Mouse Monoclonal anti-HBSAg antibody has been validated for IHC (p). It is suitable to detect HBSAg in samples from Hepatitis B Virus (HBV).

Quick Overview for HBSAg antibody (ABIN452186)

Target: HBSAg (HBsAg) (Hepatitis B Virus Surface Antigen (HBsAg))

Reactivity: Hepatitis B Virus (HBV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This HBSAg antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: P3G9A8

Product Details anti-HBSAg Antibody

Characteristics: Synonyms: HBV Large envelope protein, L glycoprotein, Large S protein, Large surface protein, HBVMajor surface antigen

Immunogen: Purified Hepatitis B surface antigen.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 1/10-1/25 (No protease pre-treatment). Blocking of endogenous Peroxidase with 1 % Peroxide in Methanol is recommended. Positive Control: HBV infected liver. Incubation Time: 60 min at RT or 2-8 °C overnight.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Stabilized Phosphate buffer, pH 7.3 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HBSAg

Target: HBSAg (HBsAg) (Hepatitis B Virus Surface Antigen (HBsAg))

Alternative Name: Hepatitis B Surface Antigen / HBsAg

Target Type: Viral Protein

Background: Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.Synonyms: HBV surface antigen, Hepatitis B Virus