M. tuberculosis antibody

Catalog No. ABIN452551

This Mouse Monoclonal antibody specifically detects M. tuberculosis in WB and EIA. It exhibits reactivity toward Mycobacterium tuberculosis.

Quick Overview for M. tuberculosis antibody (ABIN452551)

Target: M. tuberculosis (Mycobacterium Tuberculosis)

Reactivity: Mycobacterium tuberculosis

Host: Mouse

Clonality: Monoclonal

Conjugate: This M. tuberculosis antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: B12F8

Product Details anti-M. tuberculosis Antibody

Characteristics: Synonyms: M. tuberculosis, TB

Purification: Affinity Chromatography on Protein G.

Immunogen: A recombinant M. tuberculosis protein cloned from virulent strain H37Rv with primerstargeting the 38Kd antigen.

Isotype: IgG1

Application Details

Application Notes: ELISA: Reactive to the immunogen in ELISA assay. Western Blotting: A band corresponding to the immunogen is detected.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.0 without preservatives

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for M. tuberculosis

Target: M. tuberculosis (Mycobacterium Tuberculosis)

Target Type: Bacteria

Background: Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure, most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.Synonyms: M. tuberculosis, TB

Molecular Weight: 38 kDa Ag