ELK1 antibody (pSer383)

Catalog No. ABIN452640

The Mouse Monoclonal anti-ELK1 antibody (Clone B4A12) (ABIN452640) specifically detects ELK1 in EIA. The antibody is reactive with Human samples.

Quick Overview for ELK1 antibody (pSer383) (ABIN452640)

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ELK1 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Clone: B4A12

Product Details anti-ELK1 Antibody

Binding Specificity: pSer383

Specificity: Reactive to Serine383 phosphorylated Human ELK-1 peptide fragment conjugated with KLH or the peptide-BSA conjugate. Not reactive to unconjugated Serine383 phosphorylated Human ELK-1 peptide, KLH or BSA.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Elk-1, ETS domain-containing protein Elk-1

Purification: Affinity Chromatography on Protein G.

Immunogen: Peptide of a phosphorylated fragment of ELK1 (pSer383) conjugated with KLH.

Isotype: IgG1

Application Details

Application Notes: Indirect ELISA: The suggested antigen Coating quantity is 1 μg/mL of peptide/or peptideconjugating in pH 9.5 carbonate buffer, 100 μL/well.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.0 without preservatives

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ELK1

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Alternative Name: ELK1

Background: ELK1 belongs to the EST transcription factor family. Proteins in the family have a unique DNA binding domain that binds to conserved DNA sequences. Phosphorylation of ELK1 at Serine 383 and 389 by mitogen-activated protein kinases (MAPKs) activates ELK1. The binding of ELK1 to target sequences is also regulated by its co-factor, serum response factor (SRF), by alternative splicing, and by SUMOylation. ELK1 regulates the transcription of c-fos and genes encoding cytoskeleton proteins, and was found to play a role in longterm memory formation, drug addiction and other neurological disorders.Synonyms: ETS domain-containing protein Elk-1, Elk-1

Gene ID: 2002

UniProt: P19419

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling