IER5 antibody (Internal Region)

Catalog No. ABIN452683

This Goat Polyclonal antibody specifically detects IER5 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for IER5 antibody (Internal Region) (ABIN452683)

Target: IER5 (Immediate Early Response 5 (IER5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IER5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IER5 Antibody

Binding Specificity: Internal Region

Specificity: This antibody recognizes IER5.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QPPSGGEDDDAEE, from the internal region of the protein sequence according to NP_057629.1.

Application Details

Application Notes: Peptide ELISA: Antibody Detection Limit Dilution: 1/2000. Western Blot: 1-3 μg/mL. Customer's experiments gave a band at approx 45 kDa in lysatesof transfected H1299 overexpressing IER5 but not when transfected with empty vector(calculated MW of 33.5 kDa according to NP_057629.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for IER5

Target: IER5 (Immediate Early Response 5 (IER5))

Alternative Name: IER5

Background: IER5 is a similar protein to other immediate early response proteins. In mouse, a similar protein may play an important role in mediating the cellular response to mitogenic signals.Synonyms: Immediate early response gene 5 protein, PP4583, SBBI48

Gene ID: 51278

NCBI Accession: NP_057629

UniProt: Q5VY09