Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD) (AA 196-225) antibody

Catalog No. ABIN452704

This Rabbit Polyclonal antibody specifically detects in WB, IF, IHC (p), FACS and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD) (AA 196-225) antibody (ABIN452704)

Target: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Product Details

Binding Specificity: AA 196-225

Specificity: This antibody detects ACADM at Center.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide between 196~225 amino acids from the Center region of Human ACADM.

Isotype: Ig Fraction

Application Details

Application Notes: Western blot: 1: 50 - 1: 100. ELISA: 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD)

Alternative Name: ACADM

Background: ACADM protein is a medium chain specific (C4 to C12 straight chain) acyl Coenzyme A dehydrogenase. The enzyme catalyzes the initial step of the mitochondrial fatty acid beta oxidation pathway. ACADM expression is induced during periods of fasting, when reliance on fatty acids for energy is increased. Clinical phenotypes are associated with ACADM hereditary deficiency.Synonyms: MCAD, Medium-chain specific acyl-CoA dehydrogenase mitochondrial

Molecular Weight: 46588 Da

Gene ID: 34

NCBI Accession: NP_000007

UniProt: P11310