ALAD antibody (C-Term)

Catalog No. ABIN452730

This anti-ALAD antibody is a Rabbit Polyclonal antibody detecting ALAD in WB and EIA. Suitable for Human.

Quick Overview for ALAD antibody (C-Term) (ABIN452730)

Target: ALAD (Aminolevulinate Dehydratase (ALAD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALAD antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ALAD Antibody

Binding Specificity: C-Term

Specificity: This antibody detects ALAD at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide selected from the C-terminal region of human ALAD

Application Details

Application Notes: Western blot: 1: 50 - 1: 100. ELISA: 1: 1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.121 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for ALAD

Target: ALAD (Aminolevulinate Dehydratase (ALAD))

Alternative Name: ALAD

Background: The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 Molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria.Synonyms: ALADH, Delta-aminolevulinic acid dehydratase, Porphobilinogen synthase

Molecular Weight: 36295 Da

Gene ID: 210

NCBI Accession: NP_000022

UniProt: P13716