Endonuclease G antibody (C-Term)

Catalog No. ABIN452960

The Rabbit Polyclonal anti-Endonuclease G antibody has been validated for WB and EIA. It is suitable to detect Endonuclease G in samples from Human and Mouse.

Quick Overview for Endonuclease G antibody (C-Term) (ABIN452960)

Target: Endonuclease G (ENDOG)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endonuclease G antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Endonuclease G Antibody

Binding Specificity: C-Term

Specificity: This antibody detects ENDOG at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide selected from the C-terminal region of human ENDOG

Application Details

Application Notes: Western blot: 1: 50 - 1: 100. ELISA: 1: 1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for Endonuclease G

Target: Endonuclease G (ENDOG)

Alternative Name: Endonuclease G

Background: ENDOG is a nuclear encoded endonuclease that is localized in the mitochondrion. The protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.Synonyms: ENDOG

Molecular Weight: 32646 Da

Gene ID: 2021

NCBI Accession: NP_004426

UniProt: Q14249

Pathways: Apoptosis