14-3-3 gamma antibody (N-Term)

Catalog No. ABIN453478

This Rabbit Polyclonal antibody specifically detects 14-3-3 gamma in WB and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for 14-3-3 gamma antibody (N-Term) (ABIN453478)

Target: 14-3-3 gamma (YWHAG1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 gamma antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-14-3-3 gamma Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to 14-3-3 protein gamma.

Purification: Affinity chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide selected from the N-terminal region of human YWHAG

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for 14-3-3 gamma

Target: 14-3-3 gamma (YWHAG1)

Alternative Name: 14-3-3 Protein gamma

Background: YWHAG belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.Synonyms: KCIP-1, Protein kinase C inhibitor protein 1, YWHAG

Gene ID: 7532

NCBI Accession: NP_036611

UniProt: P61981

Pathways: Myometrial Relaxation and Contraction, M Phase