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ZWINT antibody (Middle Region)

This Rabbit Polyclonal antibody specifically detects ZWINT in WB, IHC (p), FACS and EIA. It exhibits reactivity toward Human and Mouse.
Catalog No. ABIN453487

Quick Overview for ZWINT antibody (Middle Region) (ABIN453487)

Target

See all ZWINT Antibodies
ZWINT (ZW10 Interactor (ZWINT))

Reactivity

  • 45
  • 5
  • 3
Human, Mouse

Host

  • 39
  • 7
Rabbit

Clonality

  • 41
  • 5
Polyclonal

Conjugate

  • 23
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This ZWINT antibody is un-conjugated

Application

  • 34
  • 13
  • 13
  • 12
  • 7
  • 7
  • 6
  • 4
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)
  • Binding Specificity

    • 15
    • 12
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Middle Region

    Specificity

    This antibody recognizes ZW10 interactor/ZWINT (Center).

    Purification

    Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

    Immunogen

    KLH conjugated synthetic peptide between 66~96 selected from the Center region of Human ZWINT Genename: ZWINT
  • Application Notes

    ELISA: 1/1,000. Western blotting: 1/50-1/100. Flow Cytometry: 1/10-1/50. Immunohistochemistry: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.25 mg/mL

    Buffer

    PBS, 0.09 % (W/V) Sodium Azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target

    ZWINT (ZW10 Interactor (ZWINT))

    Alternative Name

    ZW10 Interactor / ZWINT

    Background

    ZWINT is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells.Synonyms: ZW10-interacting protein 1, Zwint-1

    Gene ID

    11130

    NCBI Accession

    NP_001005413

    UniProt

    O95229
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