BRISC and BRCA1 A Complex Member 1 (BABAM1) (Middle Region) antibody

Catalog No. ABIN453692

The Rabbit Polyclonal anti- antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect in samples from Human.

Quick Overview for BRISC and BRCA1 A Complex Member 1 (BABAM1) (Middle Region) antibody (ABIN453692)

Target: BRISC and BRCA1 A Complex Member 1 (BABAM1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details

Binding Specificity: Middle Region

Specificity: This antibody recognizes MERIT40/HSPC142 (Center).

Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: KLH conjugated synthetic peptide selected from the Center region of Human HSPC142

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50-1/100. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: BRISC and BRCA1 A Complex Member 1 (BABAM1)

Alternative Name: MERIT40

Background: Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.Synonyms: BRCA1-A complex subunit MERIT40, C19orf62, HSPC142, Mediator of RAP80 interactions and targeting subunit of 40 kDa, NBA1, New component of the BRCA1-A complex

Gene ID: 29086

NCBI Accession: NP_001028721

UniProt: Q9NWV8

Pathways: Positive Regulation of Response to DNA Damage Stimulus