CCL2 antibody

Catalog No. ABIN453914

The Mouse Monoclonal anti-CCL2 antibody has been validated for EIA and Func. It is suitable to detect CCL2 in samples from Human.

Quick Overview for CCL2 antibody (ABIN453914)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL2 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA), Functional Studies (Func)

Clone: S382

Product Details anti-CCL2 Antibody

Specificity: This MCP1 antibody reacts with natural and recombinant Human MCP-1. Does not react with Human Interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1 beta (IL1 beta), serum Amyloid A (SAA) and epidermal growth factor (EGF).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogen: Purified recombinant Human MCP1.

Isotype: IgG2b

Application Details

Application Notes: ELISA: React with Human MCP-1. Neutralizing: Tests on the ability to inhibit monocyte chemotaxis toward 1 nM recombinanthuman MCP-1 in blindwell chambers showed that the antibodies were particularly effectiveon blocking MCP1 activity.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with double distillated water to adjust the final concentration to 1.00 mg/mL

Buffer: 0.01 M PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: MCP1 / CCL2

Background: This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.Synonyms: C-C motif chemokine 2, HC-11, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2

Gene ID: 6347

NCBI Accession: NP_002973

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection