CCL2 antibody

Catalog No. ABIN453916

This Mouse Monoclonal antibody specifically detects CCL2 in WB, EIA and Func. It exhibits reactivity toward Human.

Quick Overview for CCL2 antibody (ABIN453916)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Clone: S14

Product Details anti-CCL2 Antibody

Specificity: This MCP1 antibody reacts with natural and recombinant Human MCP-1. Does not react with Human Interleukin-8 (IL-8) and other Human cytokines tested such as Interleukin-1 beta (IL1 beta), Serum Amyloid A (SAA) and Epidermal Growth Factor (EGF).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogen: Purified recombinant Human MCP1.

Isotype: IgG1

Application Details

Application Notes: ELISA: React with Human MCP-1. Can be used as a Capture antibody in Sandwich ELISAapplications for human MCP-1 detection in combination with a monoclonaltracer/detection antibody. Suggested Capture Coating Dose: 0.3 μg/well. Substrate: TMB. If the above suggested conditions are followed approximately 1.5 pg/mL ofMCP-1 in serum/plasma or 4 pg/mL of MCP-1 in medium can be detected with an assayrange of 0-1600 pg/mL. Neutralizing: Tests on the ability to inhibit monocyte chemotaxis toward 1 nM recombinanthuman MCP-1 in blindwell chambers showed that the antibodies were particularly effectiveat blocking MCP-1 activity at a concentration of 0.5 μg/mL. It was also found that S-14 couldinhibit the function of native MCP-1 at concentrations similar to inhibitory doses forrecombinant MCP-1. Western Blot: MCP1 antibody at 0.1-1.0 μg/mL will allow visualization of 0.1 μg/lane ofhuman MCP1.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with double distillated water to adjust the final concentration to 1.00 mg/mL

Buffer: 0.01 M PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: MCP1 / CCL2

Background: This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.Synonyms: C-C motif chemokine 2, HC-11, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2

Gene ID: 6347

NCBI Accession: NP_002973

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection