PACAP antibody (C-Term)

Catalog No. ABIN453980

The Goat Polyclonal anti-PACAP antibody is suitable to detect PACAP in samples from Human, Rat, Mouse, Cow, Dog and Pig. It has been validated for WB and EIA.

Quick Overview for PACAP antibody (C-Term) (ABIN453980)

Target: PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))

Reactivity: Human, Rat, Mouse, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This PACAP antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PACAP Antibody

Binding Specificity: C-Term

Sequence: C-GKRYKQRVKN KGRR

Specificity: This antibody detects PACAP at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine, Porcine.
Species reactivity (tested):Human.

Purification: Antigen Affinity Chromatography

Immunogen: Synthetic peptide with sequence from the C-Terminus of Human ADCYAP1 (NP_001108.2). Genename: ADCYAP1

Application Details

Application Notes: Peptide ELISA: 1/2000. Western blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PACAP

Target: PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))

Alternative Name: ADCYAP1 / PACAP

Background: Adenylate cyclase activating polypeptide, mediated by adenylate cyclase activating polypeptide 1 receptors, stimulates adenylate cyclase and subsequently increases the cAMP level in target cells. Adenylate cyclase activating polypeptide 1 is not only a hypophysiotropic hormone, but also functions as a neurotransmitter and neuromodulator. In addition, it plays a role in paracrine and autocrine regulation of certain types of cells. The gene encodes three different mature peptides, including two isotypes: a shorter form and a longer form.Synonyms: Pituitary adenylate cyclase-activating polypeptide

Gene ID: 116

NCBI Accession: NP_001108

UniProt: P18509

Pathways: Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Activity, cAMP Metabolic Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Regulation of G-Protein Coupled Receptor Protein Signaling