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CD3 epsilon antibody (Activation Site) (PE)

This Mouse Monoclonal antibody specifically detects CD3 epsilon in ICFC. It exhibits reactivity toward Human and Mouse and has been mentioned in 7+ publications.
Catalog No. ABIN457363

Quick Overview for CD3 epsilon antibody (Activation Site) (PE) (ABIN457363)

Target

See all CD3 epsilon (CD3E) Antibodies
CD3 epsilon (CD3E)

Reactivity

  • 384
  • 181
  • 51
  • 43
  • 21
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  • 8
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Human, Mouse

Host

  • 243
  • 177
  • 37
  • 30
  • 23
  • 8
  • 3
  • 1
  • 1
  • 1
  • 1
Mouse

Clonality

  • 394
  • 123
  • 7
Monoclonal

Conjugate

  • 185
  • 40
  • 34
  • 24
  • 22
  • 17
  • 17
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  • 8
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  • 4
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  • 3
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  • 2
  • 2
  • 2
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  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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  • 1
This CD3 epsilon antibody is conjugated to PE

Application

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  • 1
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Intracellular Flow Cytometry (ICFC)

Clone

APA1-1
  • Binding Specificity

    • 101
    • 81
    • 26
    • 16
    • 16
    • 14
    • 11
    • 10
    • 10
    • 8
    • 8
    • 4
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    • 2
    • 2
    • 1
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    • 1
    • 1
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    • 1
    • 1
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    Activation Site

    Purpose

    Anti-CD3 epsilon (activation epitope) PE

    Specificity

    The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

    Cross-Reactivity (Details)

    Human, Mouse

    Purification

    Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

    Immunogen

    Purified human CD3 proteins isolated from thymus

    Isotype

    IgG1
  • Application Notes

    Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

    Restrictions

    For Research Use only
  • Concentration

    0.1 mg/mL

    Buffer

    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Do not freeze.
    Avoid prolonged exposure to light.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Tailor, Tsai, Shameli, Serra, Wang, Robbins, Nagata, Szymczak-Workman, Vignali, Santamaria: "The proline-rich sequence of CD3epsilon as an amplifier of low-avidity TCR signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 1, pp. 243-55, (2008) (PubMed).

    Wong, Dutly, Sacher, Lee, Hwang, Liu, Keshavjee, Hu, Waddell: "Targeted cell replacement with bone marrow cells for airway epithelial regeneration." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 293, Issue 3, pp. L740-52, (2007) (PubMed).

    Rieux-Laucat, Hivroz, Lim, Mateo, Pellier, Selz, Fischer, Le Deist: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." in: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, (2006) (PubMed).

    Gil, Schrum, Alarcón, Palmer: "T cell receptor engagement by peptide-MHC ligands induces a conformational change in the CD3 complex of thymocytes." in: The Journal of experimental medicine, Vol. 201, Issue 4, pp. 517-22, (2005) (PubMed).

    Torres, Alcover, Zapata, Arnaud, Pacheco, Martín-Fernández, Villasevil, Sanal, Regueiro: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, (2003) (PubMed).

    Delgado, Fernández, Dave, Kappes, Alarcón: "CD3delta couples T-cell receptor signalling to ERK activation and thymocyte positive selection." in: Nature, Vol. 406, Issue 6794, pp. 426-30, (2000) (PubMed).

    Alarcón, Ley, Sánchez-Madrid, Blumberg, Ju, Fresno, Terhorst: "The CD3-gamma and CD3-delta subunits of the T cell antigen receptor can be expressed within distinct functional TCR/CD3 complexes." in: The EMBO journal, Vol. 10, Issue 4, pp. 903-12, (1991) (PubMed).

  • Target

    CD3 epsilon (CD3E)

    Alternative Name

    CD3 epsilon

    Background

    CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.,CD3 epsilon chain, T3E, IMD18

    Gene ID

    916

    UniProt

    P07766

    Pathways

    TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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