beta Catenin antibody

Catalog No. ABIN457419

This anti-beta Catenin antibody is a Mouse Monoclonal antibody detecting beta Catenin in WB, IP, ICC and ICFC. Suitable for Human, Mouse and Hamster.

Quick Overview for beta Catenin antibody (ABIN457419)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Reactivity: Human, Mouse, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Catenin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC)

Clone: EM-22

Product Details anti-beta Catenin Antibody

Purpose: Anti-beta-Catenin Purified

Specificity: The mouse monoclonal antibody EM-22 reacts with C-terminal part of beta-catenin (intracellular antigen), an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription.

Cross-Reactivity (Details): Human, Mouse, Hamster

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Recombinant human beta-catenin

Isotype: IgG2a

Application Details

Application Notes: Immunocytochemistry: Positive control: HT29 human colon adenocarcinoma cell line.
Western blotting: Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line, KW1 murine cell line, C57MG murine cell line, 3T3 murine fibroblast cell line.
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Alternative Name: beta-Catenin

Background: Catenin beta 1,Beta-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta and gamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumour cells. Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of beta-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted.,CTNNB1, EVR7, MRD19

Gene ID: 1499

UniProt: P35222

Pathways: Peptide Hormone Metabolism