Sheep anti-Cow IgG1 (Fc Region) Antibody

Catalog No. ABIN457594

Quick Overview for Sheep anti-Cow IgG1 (Fc Region) Antibody (ABIN457594)

Target: IgG1

Clonality: Polyclonal

Application: Immunodiffusion (ID), Immunoelectrophoresis (IEP)

Isotype: IgG

Product Details

Binding Specificity: Fc Region

Reactivity: Cow

Host: Sheep

Specificity: The reactivity of the antiserum is directed to the subclass specific determinants on the Fc subunit of IgG1.

Characteristics: Precipitating polyclonal sheep antiserum to bovine IgG1, subclass specific
Total protein and IgG concentration in the antiserum are comparable to those of pooled normal sheep serum. Antibody titre: Precipitin titre not less then 1:16 when tested against pooled normal bovine serum in agar block

Purification: Delipidated, heat inactivated, lyophilized whole antiserum.
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Pools of purified normal IgG1 isolated from pooled bovine serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG1 in bovine serum and other body fluids or to determine its concentration.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: No foreign proteins added.

Preservative: Without preservative

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG1

Target Type: Antibody