IgA Secretory Component (Membrane-Bound), (Soluble) antibody

Catalog No. ABIN457835

The Mouse Monoclonal anti- antibody has been validated for ELISA, WB, IF and H. It is suitable to detect in samples from Human.

Quick Overview for IgA Secretory Component (Membrane-Bound), (Soluble) antibody (ABIN457835)

Target: IgA Secretory Component

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Haemagglutination (H)

Clone: NI 194-4 (A89-039)

Product Details

Binding Specificity: Membrane-Bound, Soluble

Specificity: The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.

Characteristics: Purified monoclonal mouse antibody to human secretory component, free and bound

Purification: Purified

Immunogen: Highly purified secretory component isolated from human milk.

Isotype: IgG1 kappa

Application Details

Application Notes: To identify the presence of free or bound secretory component in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining, haemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG concentration is 0.4 mg/ml. No foreign proteins added.

Buffer: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Target Details

Target: IgA Secretory Component

Alternative Name: Secretory Immunoglobulin A (SIgA)

Background: The reactivity of the antiserum is restricted to determinants on the secretory component as tested in haemagglutination, haemagglutination inhibition, indirect binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglu-tination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148).