Mouse anti-Human IgG2 Antibody (HRP)

Catalog No. ABIN457837

Quick Overview for Mouse anti-Human IgG2 Antibody (HRP) (ABIN457837)

Target: IgG2

Clonality: Monoclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA

Clone: NI 6014 (HP 6014)

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Specificity: The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab portion of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. Peroxidase conjugated purified monoclonal mouse antibody to human IgG2, subclass specific

Characteristics: Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).

Purification: Purified

Immunogen: Highly purified monoclonal IgG2 isolated from pooled human serum

Application Details

Application Notes: To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immuno-peroxidase staining of cytoplasmic IgG2, and immunoblotting.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:25 to 1:100, in ELISA from 1:250 upwards, in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Target Details

Target: IgG2

Target Type: Antibody