Lactoferrin antibody (HRP)

Catalog No. ABIN457843

This Mouse Monoclonal antibody specifically detects Lactoferrin in WB, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for Lactoferrin antibody (HRP) (ABIN457843)

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lactoferrin antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: NI 25

Product Details anti-Lactoferrin Antibody (HRP)

Specificity: The antiserum does not react with any other component of the human milk or plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.

Characteristics: Peroxidase conjugated purified monoclonal mouse antibody to human lactoferrin

Purification: Purified

Immunogen: Highly purified lactoferrin isolated from human milk.

Isotype: IgG1 kappa

Application Details

Application Notes: To identify the presence of lactoferrin in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immuno-peroxidase and indirect immunofluorescence staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards, in ELISA from 1:100 upwards, in Western blotting from 1:250 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG concentration is 0.4 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized product by addin g 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Target Details for Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Alternative Name: Lactoferrin

Background: The reactivity of the antiserum is restricted to lactoferrin as tested in indirect binding immunoblotting and indirect immunoperoxidase staining.

Pathways: Transition Metal Ion Homeostasis