IgA Secretory Component (Membrane-Bound), (Soluble) antibody (HRP)

Catalog No. ABIN457846

Product Details

Target: IgA Secretory Component

Binding Specificity: Membrane-Bound, Soluble

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Specificity: The antiserum does not react with any other component of the human milk or plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.

Characteristics: Horseradish peroxidase conjugated purified monoclonal mouse antibody to human secretory component, free and bound

Purification: Purified

Immunogen: Highly purified secretory component isolated from human milk.

Clone: NI 194-4 (A89-039)

Isotype: IgG1 kappa

Application Details

Application Notes: To identify the presence of lactoferrin in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immuno-peroxidase and indirect immunofluorescence staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards, in ELISA from 1:50 upwards, in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG concentration is 0.4 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized product by addin g 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Target Details

Target: IgA Secretory Component

Alternative Name: Secretory Immunoglobulin A (SIgA)

Background: The reactivity of the antiserum is restricted to free and bound secretory component as tested in indirect binding immunoblotting and indirect immunoperoxidase staining.