IgA Secretory Component (Membrane-Bound), (Soluble) antibody (Biotin)

Catalog No. ABIN457848

This anti- antibody is a Mouse Monoclonal antibody detecting in ELISA, WB and IF. Suitable for Human.

Quick Overview for IgA Secretory Component (Membrane-Bound), (Soluble) antibody (Biotin) (ABIN457848)

Target: IgA Secretory Component

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Clone: NI 194-4 (A89-39)

Product Details

Binding Specificity: Membrane-Bound, Soluble

Specificity: The antiserum does not react with any other component of the human milk or plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.

Characteristics: Biotin-conjugated purified monoclonal mouse antibody to human secretory component, free or bound

Purification: Purified

Immunogen: Highly purified secretory component isolated from human milk.

Isotype: IgG1 kappa

Application Details

Application Notes: To identify the presence of free or bound secretory component in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards, in ELISA from 1:25 upwards, in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG concentration is 0.4 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 1.4. No foreign proteins added.

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyoph ilized product by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Target Details

Target: IgA Secretory Component

Alternative Name: Secretory Immunoglobulin A (SIgA)

Background: The reactivity of the antiserum is restricted to secretory component as tested in indirect binding immunoblotting and indirect immunoperoxidase staining.