Mouse anti-Human IgG (Fc Region) Antibody (Biotin)

Catalog No. ABIN457881

Product Details

Target: IgG

Binding Specificity: Fc Region

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB)

Specificity: The reactivity of the antiserum is restricted to isotype specific determinants on the Fc portion of the IgG molecule. The antiserum reacts with the 4 subclasses of human IgG, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and immunoperoxidase staining of cytoplasmic Ig.

Characteristics: Biotin-conjugated purified monoclonal mouse antibody to human IgG, isotype specific
Biotin/IgG protein molar ratio (B/P) approximately 4.4.

Purification: Purified

Immunogen: Highly purified IgG isolated from pooled human serum.

Clone: NI 335 and 343

Isotype: IgG1 kappa

Application Details

Application Notes: To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 250, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Preservative: Without preservative

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody