C1S antibody

Catalog No. ABIN457973

The Goat Polyclonal anti-C1S antibody has been validated for RID, IEP and ID. It is suitable to detect C1S in samples from Human.

Quick Overview for C1S antibody (ABIN457973)

Target: C1S (Complement Component 1, S Subcomponent (C1S))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This C1S antibody is un-conjugated

Application: Radial Immunodiffusion (RID), Immunoelectrophoresis (IEP), Immunodiffusion (ID)

Product Details anti-C1S Antibody

Specificity: The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Precipitating polyclonal goat antiserum to the C1s subunit of C1 component of human complement

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: C1 component of human complement is a complex of three glycoproteins: C1q, C1r and C1s. C1s has a molecular weight of 83,000 and is present in plasma in an average concentration of 50 µ g/ml, either as a complex with C1q and C1s or in the absence of C1q as a tetramer with C1s. The subcomponents of the C1 complex are responsible for the initiation of the classical pathway of complement activation. The subunit C1s is isolated as a homogenous protein for use in antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of C1s or C1s containing complexes in human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive non-precipitating antibody-binding techniques if proper controls are included.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against in agar-block immunodiffusion titration against appropriate con

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Preservative: Without preservative

Storage: 4 °C

Target Details for C1S

Target: C1S (Complement Component 1, S Subcomponent (C1S))

Alternative Name: C1s Subunit of C1 Component of Complement

Background: The reactivity of the antiserum is restricted to C1s. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C1s protein. Complexes containing C1q may also react with the antiserum. No reaction is obtained with C1q, C1r or any other plasma protein component or serum

Pathways: Complement System