PROC antibody

Catalog No. ABIN458051

The Goat Polyclonal anti-PROC antibody has been validated for ELISA, IEP, ID, RID and Neph. It is suitable to detect PROC in samples from Human.

Quick Overview for PROC antibody (ABIN458051)

Target: PROC (Vitamin K-dependent protein C (PROC))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PROC antibody is un-conjugated

Application: ELISA, Immunoelectrophoresis (IEP), Immunodiffusion (ID), Radial Immunodiffusion (RID), Nephelometry (Neph)

Product Details anti-PROC Antibody

Specificity: The antiserum does not cross react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Precipitating polyclonal goat antiserum to human protein C

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Protein C is a glycoprotein (MW 60,000) which is synthesized in the liver. It acts as a trypsin like serine protease but functions in a manner different from that of antithrombin III. Activated protein C (pCa) results from proteolytic cleavage of protein C by thrombin and with protein S (pS) as a cofactor it cleaves factors Va and VIIIa, producing anti-coagulation by decreasing the conversion of prothrombin to thrombin. It also stimulates the fibrinolytic system by inactivating the inhibitor of tissue plasminogen activator (TPA), an enzyme that converts plasminogen into plasmin which cleaves fibrin into fibrin split products. Protein C is consumed during coagulation in vivo. Very little protein C is activated during coagulation in vitro, because this requires the presence of thrombomodulin, a thrombin receptor on endothelial cells. The coagulant activity of protein C is species specific and pCa is specifically regulated by protein C inhibitor (pCI) in the presence of heparin. Normal human plasma contains an average of 0.3 mg/ml. The normal newborn has a protein C level that is 30 to 40% of the normal adult level. Numerous cases with a genetical protein C deficiency associated with thrombotic disease have been reported, frequently with an extensive family history of thrombosis. Patients with a partial protein C deficiency are subject to severe, recurrent deep venous thrombosis and pulmonary embolism. The plasma protein C level should be determined before beginning anticoagulation therapy. Total protein C deficiency in the newborn leads to purpurea fulminans, the diagnosis may be confirmed by determining the protein C levels in both heterozygous parents. Acquired protein C deficiency may result of treatment with oral anticoagulants and of various liver diseases. Plasma protein C levels may also decrease after intravascular coagulation. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion, electro-immunodiffusion (Laurell) immunonephelometry, solid phase and neutralization assays. As catching or detection antibody in ELISA. To prepare an adsorbent for immunoaffinity purification of protein C. Plasma samples and all assay components must contain EDTA to stabilize the proteins. For electroimmunodiffusion a concentration of 1-2% of antiserum in the gel is recommended.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against pooled normal human plasma in agar-block immunodiffusion titrat

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Preservative: Without preservative

Storage: 4 °C

Target Details for PROC

Target: PROC (Vitamin K-dependent protein C (PROC))

Alternative Name: Protein C

Background: The defined antibody specificity is to human protein C as tested at the level of sensitivity of immuno-precipitation and ELISA techniques. Precipitation obtained in gel immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) using various antiserum concentrations against fresh plasma and serum shows a reaction of identity with that obtained with the purified protein C. No reaction is obtained with any other plasma or serum protein component. Complexes of protein C with protein S also react with the antiserum