Lipoprotein, beta 1 antibody

Catalog No. ABIN458082

The Rabbit Polyclonal anti-Lipoprotein, beta 1 antibody is suitable to detect Lipoprotein, beta 1 in samples from Human. It has been validated for IP.

Quick Overview for Lipoprotein, beta 1 antibody (ABIN458082)

Target: Lipoprotein, beta 1

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Immunoprecipitation (IP)

Product Details

Specificity: Precipitating polyclonal Rabbit antiSerum to Human beta-1 lipoprotein (LDL).

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.

Characteristics: The defined antibody specificity is restricted to beta-1 lipoprotein as tested at the level of sensitivity of immunoprecipitation techniques. The antiserum does not react with any other protein component of human serum or plasma. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence transferrin in human serum or other body fluids and to determine its concentration. This antiserum has not been tested in more sensitive assays as nephelometry, ELISA or use in immunohistology, but this does not exclude such use if proper controls are included.

Immunogen: Beta-1 lipoprotein has a molecular weight of 2,700,000. The protein is isolated from normal serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: In immunoelectrophoresis use 2 μL serum or equivalent against 120 μL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μL antiserum in 3 mm diameter centre well and 2 μL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added.

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Target Details

Target: Lipoprotein, beta 1

Alternative Name: beta-1 lipoproteins