Coagulation Factor IX antibody

Catalog No. ABIN458123

The Rabbit Polyclonal anti-Coagulation Factor IX antibody has been validated for IEP and ID. It is suitable to detect Coagulation Factor IX in samples from Human.

Quick Overview for Coagulation Factor IX antibody (ABIN458123)

Target: Coagulation Factor IX (F9)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Coagulation Factor IX antibody is un-conjugated

Application: Immunoelectrophoresis (IEP), Immunodiffusion (ID)

Product Details anti-Coagulation Factor IX Antibody

Specificity: The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Precipitating polyclonal rabbit antiserum to human coagulation factor IX (Christmas factor)

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Factor IX is a vitamin K-dependent glycoprotein, MW 55,400, and it consists of a single polypeptide chain. It is synthesized in the liver, stable for several days in fresh blood and for many months in fresh frozen plasma. It can be prepared as a stable concentrate. Its physicochemical properties are similar to those of several other clotting factors, which makes separation particularly difficult. Factor IX is converted to FIXa by FXIa in the presence of calcium ions. Platelets possess FXI-like activity, bypassing FXII an XI. FIXa has a molecular weight of 46,500 and is composed of two chains linked by disulphide bonds. For immunization purposes, FIX can be obtained n highly purified form from fresh plasma concentrates. After coagulation, FIX antigen is also present in serum. Abnormal molecular variants are defective in proteolytic activation and serine protease activity, while calcium binding is also impaired. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as electroimmunodiffusion, immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony). To prepare an adsorbent for immunoaffinity purification of FIX. If used in more sensitive test procedures or as catching or detection antibody in solid phase immunoassays specificity controls should always be include. Plasma samples and all assay components must contain EDTA to stabilize the proteins. Normal adult plasma contains an average of 1 mg FIX per 100 ml. In a normal population this varies between 80% and 120% of the average. Newborn infants have a level of about 75% of the average adult level. Absence of FIX in plasma causes a bleeding diathesis very similar to haemophilia A in clinical manifestations and from the point of view of heredity. It is known as haemophilia B or Christmas disease. Different types have been described. The most common type demonstrates reduced FIX coagulant activity without measurable FIX relates antigen. Other types of reduced FIX antigen and coagulation activity, or reduced coagulant activity with normal antigen levels. Heterozygote carriers can be detected. Acquired depression of plasma FIX levels are seen in vitamin K deficiencies, in liver disease, or following therapy with vitamin K antagonists (oral coagulants).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 when tested against appropriate concentrations of the antigen in ag

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged stora ge at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Target Details for Coagulation Factor IX

Target: Coagulation Factor IX (F9)

Alternative Name: Coagulation Factor IX (Christmas Factor)

Background: The defined antibody reactivity is restricted to Factor IX as tested at the level of sensitivity of immuno-precipitation techniques. A single precipitin line is obtained in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with the purified immunogen. No precipitation is obtained with any other plasma protein and with haemophilia B plasma