Complement C3b antibody

Catalog No. ABIN458141

The Rabbit Polyclonal anti-Complement C3b antibody has been validated for IEP. It is suitable to detect Complement C3b in samples from Human.

Quick Overview for Complement C3b antibody (ABIN458141)

Target: Complement C3b (C3b) (Complement C3 beta Chain (C3b))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Complement C3b antibody is un-conjugated

Application: Immunoelectrophoresis (IEP)

Product Details anti-Complement C3b Antibody

Specificity: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Precipitating polyclonal rabbit antiserum to C3b subunit of human complement C3

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: C3b (C3c+C3d) has a molecular weight of 170,000. The protein is isolated and purified from pooled normal human serum by precipitation techniques, followed by chromatographical methods. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of complement C3b or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:80 when tested against normal human plasma in agar-block immunodiffusi

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Target Details for Complement C3b

Target: Complement C3b (C3b) (Complement C3 beta Chain (C3b))

Alternative Name: C3b Subunit of Complement C3

Background: In immunoelectrophoresis and radial immunodiffusion, using various antiserum concen-trations against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. The antiserum does not react with any other protein component of human serum or plasma

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling