Goat anti-Human IgG (Fc Region) Antibody - Preadsorbed

Catalog No. ABIN458217

Quick Overview for Goat anti-Human IgG (Fc Region) Antibody - Preadsorbed (ABIN458217)

Target: IgG

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Isotype: IgG

Product Details

Binding Specificity: Fc Region

Reactivity: Human

Host: Goat

Specificity: The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule including all subclasses. In immunoelectrophoresis and double radial immunodiffusion, using various anti-serum concentrations against normal human plasma and serum, a characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG.

Characteristics: Purified IgG fraction of polyclonal goat antiserum to human IgG, Fc specific
Antibody titre: Precipitin titre not less than 1:32 when tested against normal human serum in agar block titration.

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.

Immunogen: Purified normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:2,500.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Handling Advice: Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG

Target Type: Antibody