Lactoferrin antibody

Catalog No. ABIN458317

This Goat Polyclonal antibody specifically detects Lactoferrin in WB, IHC, ELISA and ICC. It exhibits reactivity toward Monkey.

Quick Overview for Lactoferrin antibody (ABIN458317)

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivity: Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Lactoferrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Product Details anti-Lactoferrin Antibody

Specificity: The antiserum does not cross-react with any other monkey plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail, however a strong cross reaction with lactoferrin in human milk has been observed.

Characteristics: Purified IgG fraction of polyclonal goat antiserum to monkey lactoferrin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized fractions of monkey serum and lactoferrin-depleted monkey milk as required, to eliminate antibodies reacting with other monkey serum or milk proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. The IgG (7S) fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.

Immunogen: Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight anti-microbial action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from rhesus monkey milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In enzyme-immunocytochemical and immunohistochemical techniques for the indirect detection of monkey lactoferrin at the cellular and subcellular level in appropriately treated cell and tissue substrates, as catching reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). The optimum working dilution of the product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:500.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. No foreign proteins added.

Buffer: Purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)

Preservative: Without preservative

Storage: 4 °C

Target Details for Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Alternative Name: Lactoferrin

Background: Tested in immunoelectrophoresis against monkey milk a single precipitin line is obtained. The antiserum does not react with any other protein component of monkey serum or plasma

Pathways: Transition Metal Ion Homeostasis