alpha 2 Macroglobulin antibody

Catalog No. ABIN458423

The Rabbit Polyclonal anti-alpha 2 Macroglobulin antibody has been validated for IEP and ID. It is suitable to detect alpha 2 Macroglobulin in samples from Mouse.

Quick Overview for alpha 2 Macroglobulin antibody (ABIN458423)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha 2 Macroglobulin antibody is un-conjugated

Application: Immunoelectrophoresis (IEP), Immunodiffusion (ID)

Product Details anti-alpha 2 Macroglobulin Antibody

Specificity: Inter-species cross reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has been tested in double radial immunodiffusion (Ouchterlony) with the following results: bovine ± cat ± chicken - dog - ferret ± goat - guinea pig ± hamster + horse ± human - monkey - pigeon - rat ++ sheep - swine ± turkey -

Characteristics: Precipitating polyclonal rabbit antiserum to mouse alpha-2 macroglobulin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Alpha-2 macroglobulin ids the major protein in the alpha-2 region of mouse serum after separation by electrophoresis. It is a large polymer and a strong antigen. It forms about one-third in the total of the alpha-2 globulins. The protein is isolated by ammonium sulphate precipitation, ion exchange chromatography, gel filtration and preparative starch block electrophoresis. In the purification of alpha-2 macroglobulin the activation of kallikrein is avoided, since it forms an irreversible bond with aplpha-2 macroglobulin, thus contaminating the final immunogen. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence alpha-2 macroglobulin in mouse serum or other body fluids and to determine its con-centration. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays, but this does not exclude such use if proper controls are included.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against pooled normal mouse sera of different strains in agar-block i

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Target Details for alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: alpha-2 Macroglobulin

Background: The reactivity of the antiserum is restricted to alpha-2 macroglobulin as tested in immuno-electrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified alpha-2 macroglobulin

Pathways: Lipid Metabolism