Rabbit anti-Mouse IgA, IgG, IgM (Fab Region) Antibody - Preadsorbed

Catalog No. ABIN458528

Quick Overview for Rabbit anti-Mouse IgA, IgG, IgM (Fab Region) Antibody - Preadsorbed (ABIN458528)

Target: IgA, IgG, IgM

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC)

Isotype: IgG

Product Details

Binding Specificity: Fab Region

Reactivity: Mouse

Host: Rabbit

Specificity: The defined antibody specificity is directed to the major isotypes of the mouse immunoglobulin system (classes, subclasses and L chain types) including antibody to common determinants, to class and subclass-specific determinants, and to the surface determinants of the common Fab portion as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony).

Characteristics: Purified IgG fraction of polyclonal rabbit antiserum to mouse immunoglobulins IgG, IgA, IgM, heavy and light chains, adsorbed for human cross-reactivity.

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized Ig-depleted mouse.

Immunogen: Highly purified IgG, including all subclasses, and pools of homogenous IgA and IgM isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted.
The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays are between 1:500 and 1:5,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Handling Advice: Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG + IgA + IgM

Target Type: Antibody