alpha 2 Macroglobulin antibody

Catalog No. ABIN458636

The Goat Polyclonal anti-alpha 2 Macroglobulin antibody has been validated for IEP. It is suitable to detect alpha 2 Macroglobulin in samples from Rat.

Quick Overview for alpha 2 Macroglobulin antibody (ABIN458636)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This alpha 2 Macroglobulin antibody is un-conjugated

Application: Immunoelectrophoresis (IEP)

Product Details anti-alpha 2 Macroglobulin Antibody

Specificity: The antiserum does not cross-react with any other component of rat plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail, however in double radial immunodiffusion a reaction has been observed with mouse.

Characteristics: Precipitating polyclonal goat antiserum to rat alpha-2 macroglobulin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Alpha-2 macroglobulin(clotting factor I) is the major protein in the alpha-2 region of rat serum after separation by immunoelectrophoresis. The concentration in normal plasma is about 2.5 mg/ml. It is a large polymer and a strong antigen. It forms about one third in the total of the alpha-2 globulins. The protein is isolated by ammonium sulphate precipitation, ion exchange chromatography, gelfiltration and preparative starch block electrophoresis. In the purification the activation of kallikrein is avoided, since it forms an irreversible bond with alpha-2 macroglobulin, thus contaminating the final immunogen. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and single or double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of alpha-2 macroglobulin in rat plasma or other body fluids or to determine its concentration. This antiserum is not intended for use in non-precipitating antibody binding or other highly sensitive assays, but this does exclude such use if proper controls are included.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:8 when tested against pooled normal rat plasma in agar-block immunodiffusion titration

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Preservative: Without preservative

Storage: 4 °C

Target Details for alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: alpha-2 Macroglobulin

Background: The reactivity of the antiserum is restricted to rat alpha-2 macroglobulin. In immunoelectro-phoresis and radial immunodiffusion, using various antiserum concentrations against normal rat plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified alpha-2 macroglobulin. No reaction is obtained with any other plasma protein components or serum

Pathways: Lipid Metabolism