The Rabbit Polyclonal anti-Amyloglucosidase antibody has been validated for IF, WB and ELISA. It is suitable to detect Amyloglucosidase in samples from Aspergillus niger.
Catalog No. ABIN458876
Quick Overview for Amyloglucosidase antibody (ABIN458876)
Target
Amyloglucosidase
Reactivity
Aspergillus niger
Host
Rabbit
Clonality
Polyclonal
Conjugate
This Amyloglucosidase antibody is un-conjugated
Application
Immunofluorescence (IF), Western Blotting (WB), ELISA
Specificity
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Characteristics
Affinity purified antibodies of polyclonal rabbit antiserum to amyloglucosidase from Aspergillus niger
Purification
Specific polyclonal antibody fractions were prepared by solid phase affinity chromatography.
Immunogen
Amyloglucosidase isolated and purified from Aspergillus niger. Freund’s complete adjuvant is used in the first step of the immunization procedure.
This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
Restrictions
For Research Use only
Format
Lyophilized
Concentration
PAb concentration 1 mg/0.5ml. No foreign proteins added.
Buffer
Purified hyperimmune rabbit IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran.
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Target
Amyloglucosidase
Background
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.