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DNASE1 antibody

The Rabbit Polyclonal anti-DNASE1 antibody has been validated for WB, ELISA and IF. It is suitable to detect DNASE1 in samples from Cow.
Catalog No. ABIN458900

Quick Overview for DNASE1 antibody (ABIN458900)

Target

See all DNASE1 Antibodies
DNASE1 (Deoxyribonuclease I (DNASE1))

Reactivity

  • 70
  • 26
  • 16
  • 16
Cow

Host

  • 68
  • 17
  • 1
Rabbit

Clonality

  • 71
  • 15
Polyclonal

Conjugate

  • 44
  • 12
  • 9
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This DNASE1 antibody is un-conjugated

Application

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  • 15
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  • 11
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Western Blotting (WB), ELISA, Immunofluorescence (IF)
  • Specificity

    Cross-reactivities against enzymes of other sources may occur but have not been determined.

    Characteristics

    Affinity purified antibodies of polyclonal rabbit antiserum to deoxyribonuclease I from bovine pancreas

    Purification

    Specific polyclonal antibody fractions were prepared by solid phase affinity chromatography.

    Immunogen

    Deoxyribonuclease I isolated and purified from bovine pancreas. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Application Notes

    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Concentration

    PAb concentration 1 mg/0.5ml. No foreign proteins added.

    Buffer

    Purified hyperimmune rabbit IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran.

    Preservative

    Without preservative

    Storage

    4 °C/-20 °C

    Storage Comment

    The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Target

    DNASE1 (Deoxyribonuclease I (DNASE1))

    Alternative Name

    Deoxyribonuclease I

    Background

    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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